An eco-friendly approach to enhance the extraction and recovery efficiency of isoflavones from kudzu roots and soy molasses wastes using ultrasound-assisted extraction with natural deep eutectic solvents (NADES)

The extraction efficiency, recovery, quantification, and stability of isoflavones (daidzein, genistein puerarin) from the wastes /by-products kudzu roots soy molasses were evaluated by using natural deep eutectic solvents (NADESs) coupled with ultrasound-assisted extraction. NADES was compared conventional Soxhlet technique, extracted quantified HPLC-UV/Vis. Results showed extractability puerarin to be significantly increased (p < 0.0001) in proportion water concentration (9.7 ± 0.2, 12.6 14.8 0.3 mg/g NADESs containing 10%, 20%, 30% water, respectively). Despite having less isoflavone content, extracts obtained exhibited higher antioxidant activity when methanolic extracts. Further, also markedly reduced degradation degree isoflavones. results this investigation support use green techniques obtain value-added components like wastes/by-products that molasses, which are envisaged find future potential applications food pharmaceutical industries.